pubseqos.txt
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- Update of PubSeqOS interface from Michael Kimelman at 12/19/2003.
- 1. id_gi_by_seqid_asn
- this call now return sat, sat_key and external features mask for any resolvable
- Seqid.
- keep in mind that it also recognises constructions like
- Seq-id ::= general { db "NCBI" , tag id 2 }'
- which is actually a reference to whole blob (sat=4,sat_key=2) rather than specific
- sequence in it.
- PUBSEQ_OS.kimelman.|20.1> id_gi_by_seqid_asn 'Seq-id ::= gi 6';
- gi sat sat_key extra_feat
- ----------- ------ ----------- -----------
- 6 2 4197975 0
- PUBSEQ_OS.kimelman.|27.1> id_gi_by_seqid_asn 'Seq-id ::= general { db "TRACE" ,
- tag id 2 }';
- gi sat sat_key extra_feat
- ----------- ------ ----------- -----------
- 0 28 2 0
- PUBSEQ_OS.kimelman.|28.1> id_gi_by_seqid_asn 'Seq-id ::= general { db "ti" , tag
- id 2 }';
- gi sat sat_key extra_feat
- ----------- ------ ----------- -----------
- 0 28 2 0
- PUBSEQ_OS.kimelman.|37.1> id_gi_by_seqid_asn 'Seq-id ::= general { db "ANNOT:CDD"
- , tag id 6 }';
- gi sat sat_key extra_feat
- ----------- ------ ----------- -----------
- 6 26 6 0
- 2. show_annotations
- this call allows you to retrieve the interpretation of extra_feat mask.
- you need to run it once for the life of connection. (or refresh it once in a
- while)
- PUBSEQ_OS.kimelman.|29.1> show_annotations ;
- name annot_bit sat internal
- --------------- ----------- ------ -----------
- SNP 1 26 0
- 3. id_get_asn
- this new satellite 26 provides annotation in different packaging.
- they are packaged as a regular Seq-entry with seqid like
- Seqid ::= general { db "ANNOT:<annot_name>" , tag id <gi> }
- you can check id_get_asn 6,6,26,0,1,0,0,0,8; as example.
- Seq-entry ::= seq {
- id { general { db "Annot:CDD" , tag id 6 } } ,
- inst {
- repr delta , mol aa , length 342 , topology not-set ,
- ext delta {
- loc int { from 0 , to 341 , id gi 6 } } } ,
- annot { ....
- }}
- you also can get new values of zip_type field in first result set of id_get_asn.
- PUBSEQ_OS.kimelman.|36.1> id_get_asn 6,6,26,0,0,0,0,0,8;
- sat_key sat state confidential suppress override username div class hup_date
- zip_type last_touched_m
- ----------- ------ ----- ------------ -------- -------- -------- --- -----
- ---------------
- -------- --------------
- 6 26 100 0 0 0 kimelman N/A 0 1/1/1900
- 2 17823575
- the old values was:
- 0: nothing special : plain asn1 (or smth else according to outfmt (fifth argument of
- id_get_asn))
- 1: nlmzipped SNP annotation.
- new values:
- 2: bit flag - output is gzipped.
- you will get zip_type&2>0 only if you informed pubseq that you can accept
- gzipped
- data. by
- PUBSEQ_OS.kimelman.|35.1> set accept gzip;
- 4: bit_flag - output contains more than one seq-entry wrapped in
- Seq-entry ::= set { class genbank, set { <set of seqentries> } }
- currently you can get this kind of output only if you request more than one
- external annotation for gi which actually has more than one.